LOCUS       sequence-73737-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows co-translational incorporation of the fluorescent ncAA Anap 
            directly into the target protein; expresses amber suppressor tRNA 
            and the synthetase AnapRS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Chatterjee A, Guo J, Lee HS, Schultz PG
  TITLE     A genetically encoded fluorescent probe in Mammalian cells.
  JOURNAL   J Am Chem Soc. 2013 Aug 28;135(34):12540-3. doi: 10.1021/ja4059553. 
            Epub 2013 Aug 14.
  PUBMED    23924161
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja4059553";
            journalName: "J Am Chem Soc"; date: "2013-08-28- 28"; volume: "135";
            issue: "34"; pages: "12540-3"
COMMENT     Sequence Label: pANAP
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     tRNA            complement(join(1..47,48..50,51..84))
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        complement(89..183)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            complement(join(197..243,244..246,247..280))
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        complement(285..379)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    285..303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    313..331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            complement(join(393..439,440..442,443..476))
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        complement(481..575)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    481..499
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    509..527
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     tRNA            complement(join(589..635,636..638,639..672))
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     protein_bind    677..695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    705..723
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tacccggagc gggacttgaa cccgcacagc gcgaacgccg agggatttag aatcccttgt
       61 gtctaccgat tccaccatcc gggcggtgtc tctatcactg atagggaact tataagtctc
      121 tatcactgat agggatttca cgtttatggt gatttcccag aacacatagc gacatgcaaa
      181 tattagatcc aaaaaatacc cggagcggga cttgaacccg cacagcgcga acgccgaggg
      241 atttagaatc ccttgtgtct accgattcca ccatccgggc ggtgtctcta tcactgatag
      301 ggaacttata agtctctatc actgataggg atttcacgtt tatggtgatt tcccagaaca
      361 catagcgaca tgcaaatatt agatccaaaa aatacccgga gcgggacttg aacccgcaca
      421 gcgcgaacgc cgagggattt agaatccctt gtgtctaccg attccaccat ccgggcggtg
      481 tctctatcac tgatagggaa cttataagtc tctatcactg atagggattt cacgtttatg
      541 gtgatttccc agaacacata gcgacatgca aatattagat ccaaaaaata cccggagcgg
      601 gacttgaacc cgcacagcgc gaacgccgag ggatttagaa tcccttgtgt ctaccgattc
      661 caccatccgg gcggtgtctc tatcactgat agggaactta taagtctcta tcactgatag
      721 ggatttcacg tttatggtga tttcccag
//
