LOCUS       sequence-73746-e        5313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses EGFP-Rab10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Rzomp KA, Scholtes LD, Briggs BJ, Whittaker GR, Scidmore MA
  TITLE     Rab GTPases are recruited to chlamydial inclusions in both a 
            species-dependent and species-independent manner.
  JOURNAL   Infect Immun. 2003 Oct;71(10):5855-70.
  PUBMED    14500507
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2003-10"; volume: "71(10)"; pages: "5855-70"
COMMENT     Sequence Label: EGFP-Rab10
FEATURES             Location/Qualifiers
     source          1..5313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    762..883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(799..818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     853..872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(890..1345)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1027..1048)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1239..1258
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1372..1476
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1478..1835
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1478..1669
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1501..1521)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1686..1821
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1870..2664
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1870..2664
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1924..1943)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2534..2553
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2852..2871)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2896..2943
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3272..3860
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3761..3780
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4035..4338
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4339..4542
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4493..4513
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4581..4590
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4587..4589,4590..4592,4593..5303)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4587..4589,4590..4592,4593..5303)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4632..4653)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4893..4912)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5240..5261
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccaccat ggcgaagaag acgtacgacc tgcttttcaa gctgctcctg atcggggatt
       61 ccggagtggg gaagacctgc gtcctttttc gtttttcgga tgatgccttc aatactacct
      121 ttatttccac cataggaata gacttcaaga tcaaaacagt tgaattacaa ggaaagaaga
      181 tcaagctaca gatatgggat acagcaggcc aggagcgatt tcacaccatc acaacctcct
      241 actacagagg cgcaatgggt atcatgctag tatatgacat caccaatggt aaaagttttg
      301 aaaacatcag caaatggctt agaaacatag atgagcatgc caatgaagat gtggaaagaa
      361 tgttactagg aaacaagtgt gatatggacg acaaaagagt tgtacctaaa ggaaaaggag
      421 aacagattgc aagggagcat ggtattaggt tttttgagac tagtgcaaaa gcaaatataa
      481 acatcgaaaa ggcgttcctc acgttagctg aagatatcct tcgaaagacc cctgtaaaag
      541 agcccaacag tgaaaatgta gatatcagca gtggaggagg cgtgacaggc tggaagagca
      601 aatgctgctg actcgacggt accgcgggcc cgggatccac cggatctaga taactgatca
      661 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
      721 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      781 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      841 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc
      901 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      961 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     1021 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     1081 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     1141 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     1201 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     1261 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     1321 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1381 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1441 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
     1501 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     1561 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     1621 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     1681 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     1741 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     1801 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga
     1861 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1921 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1981 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     2041 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     2101 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     2161 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     2221 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     2281 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     2341 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
     2401 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     2461 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     2521 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     2581 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     2641 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     2701 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     2761 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     2821 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     2881 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg
     2941 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     3001 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     3061 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact
     3121 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3181 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3241 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3301 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3361 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     3421 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3481 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3541 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3601 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3661 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3721 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3781 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3841 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3901 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3961 ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4021 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4081 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     4141 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     4201 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     4261 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     4321 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     4381 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     4441 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     4501 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     4561 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     4621 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     4681 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     4741 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     4801 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4861 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4921 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4981 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     5041 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     5101 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5161 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     5221 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5281 tcggcatgga cgagctgtac aagtccggac tca
//
