LOCUS       sequence-73806-e        2907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative feedback plasmid from oscillator without luxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J
  TITLE     A sensing array of radically coupled genetic 'biopixels'.
  JOURNAL   Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
  PUBMED    22178928
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-12-18- 18"; volume: "481"; issue: "7379"; 
            pages: "39-44"
COMMENT     Sequence Label: pTD103aiiA(Cm) -R
FEATURES             Location/Qualifiers
     source          1..2907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             197..208
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1018..1104
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1139..1156)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1273..1818)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1353..1372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1932..2026)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2047..2706)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2621..2640
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2707..2809)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2875..2893
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagacct attgtttgtc gcaagttttg cgtgttatat atcattaaaa cggtaatgga
       61 ttgacatttg attctaataa attggatttt tgtcacacta ttgtatcgct gggaatacaa
      121 ttacttaaca taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata
      181 gtcgaatgaa ttcattaaag aggagaaagg taccatgaca gtaaagaaac tttatttcat
      241 cccagcaggt cgttgcatgt tggatcattc gtctgttaac agtgcgttaa caccggggaa
      301 actattaaac ttgccggtgt ggtgttatct tttggagacg gaagaaggtc ctattttagt
      361 agacacaggt atgccagaaa gtgcagttaa taatgaaggg ctttttaacg gtacatttgt
      421 tgaaggacag atcttaccga aaatgactga agaagataga atcgtgaata tattaaagcg
      481 tgtagggtat gagccggacg accttttata tattattagt tctcacttac attttgatca
      541 tgcaggagga aacggtgctt ttacaaatac accaattatt gtgcagcgaa cggaatatga
      601 ggcagcactt catagagaag aatatatgaa agaatgtata ttaccgcatt tgaactacaa
      661 aattattgaa ggggattatg aagtggtacc aggtgttcaa ttattgtata cgccaggtca
      721 ttctccaggc catcagtcgc tattcattga gacggagcaa tccggttcag ttttattaac
      781 gattgatgca tcgtacacga aagagaattt tgaagatgaa gtgccgttcg caggatttga
      841 tccagaatta gctttatctt caattaaacg tttaaaagaa gttgtgaaaa aagagaaacc
      901 aattattttc tttggtcatg atatagagca ggaaaagagt tgtagagtgt tcccggaata
      961 tatagcagcg aacgacgaaa attacgccct tgcagcgtaa acgcgtgcta gaggcatcaa
     1021 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1081 aacgctctcc tgagtaggac aaatccgccg ccctagacct agggatatat tccgcttcct
     1141 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac
     1201 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
     1261 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     1321 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
     1381 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     1441 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     1501 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
     1561 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
     1621 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
     1681 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
     1741 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
     1801 agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca
     1861 gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtta
     1921 ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1981 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     2041 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     2101 gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac
     2161 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     2221 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     2281 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     2341 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     2401 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     2461 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
     2521 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     2581 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     2641 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2701 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     2761 tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt
     2821 tcgccagata tcgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2881 cgtatcacga ggccctttcg tcttcac
//
