LOCUS       sequence-73810-e        4836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ArsR-regulated expression of luxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J
  TITLE     A sensing array of radically coupled genetic 'biopixels'.
  JOURNAL   Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
  PUBMED    22178928
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-12-18- 18"; volume: "481"; issue: "7379"; 
            pages: "39-44"
COMMENT     Sequence Label: pZSm47a luxR RBS
FEATURES             Location/Qualifiers
     source          1..4836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             596..607
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1384..1470
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1969..2191
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2239..3189
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3720..3814)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3826..4617)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gacgtctttc caagttatct cacctacctt aaggtaatag tgtgattaat catatgcgtt
       61 tttggttatg tgttgtttga cttaatatca gagccgagag atacttgttt tctacaaagg
      121 agagggaaat gttgcaacta acaccacttc agttatttaa aaacctgtcc gatgaaaccc
      181 gtttgggtat cgtgttgttg ctcagggaga tgggagagtt gtgcgtgtgt gatctttgca
      241 tggcactgga tcaatcacag cccaaaatat cccgtcatct ggcgatgcta cgggaaagtg
      301 gaatccttct ggatcgtaaa cagggaaaat gggttcacta ccgcttatca ccgcatattc
      361 cttcatgggc tgcccagatt attgagcagg cctggttaag ccaacaggac gacgttcagg
      421 tcatcgcacg caagctggct tcagttaact gctccggtag cagtaaggct gtctgcatct
      481 aaaaaatttg cctgaacata tatgttttat caaatgcgag gtatttaaga tgaaaacgtt
      541 aatggtattt gacccggcga tgtgttgcag caccggtatc actagagaat tcattaaaga
      601 ggagaaaggt accatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat
      661 taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt
      721 acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat
      781 ttcaattcta gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat
      841 aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat
      901 atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc
      961 aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct
     1021 tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat
     1081 gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa
     1141 taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga
     1201 aggaaaaagc tcttgggata tttcaaaaat attaggctgc agtgagcgta ctgtcacttt
     1261 ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa
     1321 agcaatttta acaggagcaa ttgattgccc atactttaaa aattaaacgc gtgctagagg
     1381 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1441 tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacctaggg acagtaagac
     1501 gggtaagcct gttgatgata ccgctgcctt actgggtgca ttagccagtc tgaatgacct
     1561 gtcacgggat aatccgaagt ggtcagactg gaaaatcaga gggcaggaac tgctgaacag
     1621 caaaaagtca gatagcacca catagcagac ccgccataaa acgccctgag aagcccgtga
     1681 cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt
     1741 gccatttacc cccattcact gccagagccg tgagcgcagc gaactgaatg tcacgaaaaa
     1801 gacagcgact caggtgcctg atggtcggag acaaaaggaa tattcagcga tttgcccgag
     1861 cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc tgttttgtag aggagcaaac
     1921 agcgtttgcg acatcctttt gtaatactgc ggaactgact aaagtagtga gttatacaca
     1981 gggctgggat ctattctttt tatctttttt tattctttct ttattctata aattataacc
     2041 acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg aatttacaga gggtctagca
     2101 gaatttacaa gttttccagc aaaggtctag cagaatttac agatacccac aactcaaagg
     2161 aaaaggacaa gtaattatca ttgactagcc catctcaatt ggtatagtga ttaaaatcac
     2221 ctagaccaat tgagatgtat gtctgaatta gttgttttca aagcaaatga actagcgatt
     2281 agtcgctatg acttaacgga gcatgaaacc aagctaattt tatgctgtgt ggcactactc
     2341 aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg tatcgttcac ttataaccaa
     2401 tacgctcaga tgatgaacat cagtagggaa aatgcttatg gtgtattagc taaagcaacc
     2461 agagagctga tgacgagaac tgtggaaatc aggaatcctt tggttaaagg ctttaagatt
     2521 ttccagtgga caaactatgc caagttctca agcgaaaaat tagaattagt ttttagtgaa
     2581 gagatattgc cttatctttt ccagttaaaa aaattcataa aatataatct ggaacatgtt
     2641 aagtcttttg aaaacaaata ctctatgagg atttatgagt ggttattaaa agaactaaca
     2701 caaaagaaaa ctcacaaggc aaatatagag attagccttg atgaatttaa gttcatgtta
     2761 atgcttgaaa ataactacca tgagtttaaa aggcttaacc aatgggtttt gaaaccaata
     2821 agtaaagatt taaacactta cagcaatatg aaattggtgg ttgataagcg aggccgcccg
     2881 actgatacgt tgattttcca agttgaacta gatagacaaa tggatctcgt aaccgaactt
     2941 gagaacaacc agataaaaat gaatggtgac aaaataccaa caaccattac atcagattcc
     3001 tacctacgta acggactaag aaaaacacta cacgatgctt taactgcaaa aattcagctc
     3061 accagttttg aggcaaaatt tttgagtgac atgcaaagta agcatgatct caatggttcg
     3121 ttctcatggc tcacgcaaaa acaacgaacc acactagaga acatactggc taaatacgga
     3181 aggatctgag gttcttatgg ctcttgtatc tatcagtgaa gcatcaagac taacaaacaa
     3241 aagtagaaca actgttcacc gttagatatc aaagggaaaa ctgtccatat gcacagatga
     3301 aaacggtgta aaaaagatag atacatcaga gcttttacga gtttttggtg catttaaagc
     3361 tgttcaccat gaacagatcg acaatgtaac agatgaacag catgtaacac ctaatagaac
     3421 aggtgaaacc agtaaaacaa agcaactaga acatgaaatt gaacacctga gacaacttgt
     3481 tacagctcaa cagtcacaca tagacagcct gaaacaggcg atgctgctta tcgaatcaaa
     3541 gctgccgaca acacgggagc cagtgacgcc tcccgtgggg aaaaaatcat ggcaattctg
     3601 gaagaaatag cgctttcagc cggcaaaccg gctgaagccg gatctgcgat tctgataaca
     3661 aactagcaac accagaacag cccgtttgcg ggcagcaaaa cccgtacact agtgcttgga
     3721 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     3781 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcttatt tgccgactac
     3841 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
     3901 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
     3961 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
     4021 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
     4081 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
     4141 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
     4201 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
     4261 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
     4321 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
     4381 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
     4441 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
     4501 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
     4561 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgat
     4621 gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
     4681 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
     4741 cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg
     4801 ttcggtcaag gttctggacc agttgcgtga gcgcat
//
