LOCUS       sequence-73843-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir50-7
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    48..72
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(250..274)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             627..668
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(708..727)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    752..800
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 aatacgactc actataggga gacccaagct ggctagttaa gctatcaaca agtttgtaca
       61 aaaaagcagg ctctattttt actaattctg aattccataa ttctatattc tgtattcctc
      121 cccgccggcc gctgatatgt ctggtattct tgggtttgaa cttccagcgt tgaacccgca
      181 tattagacgt atcgacggcc ggcggggcat actgctaagt ttggagagac cgggggagtc
      241 ccagagatgc cccagctttc ttgtacaaag tggttgatct agagggcccg cagtggggtc
      301 agcttctacc ggggcggcgt cccggggtct tggaactgca tgcagactgc ctgcttggga
      361 aacatacttc tttatatgcc catatggacc tgctaagcta tggaatgtaa agaagtatgt
      421 atctcaggcc gggacctctc tcgccgcact gaggggcact ccacaccacg ggggccgccg
      481 cggccgacag ccagccaggc ggctgtggtg ggggtgaggc tggggcggca ggctgcctgg
      541 gcgttggtgg acggtcgtgt ccccgccagc attgttctgt cactttgcct gtgtcagcag
      601 gtggaagtgc tcaccagaca ttcgaaggta agcctatccc taaccctctc ctcggtctcg
      661 attctacgcg taccggttag taatgagttt aaacggggga ggctaactga aacacggaag
      721 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
      781 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
      841 taccccaccg aga
//
