LOCUS       sequence-73861-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir50-wt
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    50..74
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(258..282)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             635..676
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(716..735)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    760..808
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttaatacgac tcactatagg gagacccaag ctggctagtt aagctatcaa caagtttgta
       61 caaaaaagca ggctctattt ttactaattc tgaattccat aattctatat tctgtattcc
      121 tgcccgccgg ccgctgatat gtctggtatt cttgggtttg aacttccagc gttgaacccg
      181 catattagac gtatcgacgg ccggcggggc aggtaatggc tggtaagttt ggagagaccg
      241 ggggagtccc agagatgccc cagctttctt gtacaaagtg gttgatctag agggcccgca
      301 gtggggtcag cttctaccgg ggcggcgtcc cggggtcttg gaactgcatg cagactgcct
      361 gcttgggaaa catacttctt tatatgccca tatggacctg ctaagctatg gaatgtaaag
      421 aagtatgtat ctcaggccgg gacctctctc gccgcactga ggggcactcc acaccacggg
      481 ggccgccgcg gccgacagcc agccaggcgg ctgtggtggg ggtgaggctg gggcggcagg
      541 ctgcctgggc gttggtggac ggtcgtgtcc ccgccagcat tgttctgtca ctttgcctgt
      601 gtcagcaggt ggaagtgctc accagacatt cgaaggtaag cctatcccta accctctcct
      661 cggtctcgat tctacgcgta ccggttagta atgagtttaa acgggggagg ctaactgaaa
      721 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
      781 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg
//
