LOCUS       sequence-73866-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechnol J. 2008 Oct;3(9-10):1280-5. doi: 10.1002/biot.200800140.
  PUBMED    18729046
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2008-10"; volume: "3(9-10):1280-5. doi"; pages: " 
            10.1002/biot.200800140"
COMMENT     Sequence Label: pFA6a-5FLAG-hphMX6
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..182
                     /codon_start=1
                     /product="five tandem FLAG(R) epitope tags"
                     /label=5xFLAG
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     terminator      209..396
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(264..288)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        471..814
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 aagcttcgta cgctgcaggt cgacggatcc ccgggttaat taatcatatg gactacaagg
       61 acgacgatga caaggattac aaagatgacg acgataagct tatggactac aaggacgacg
      121 atgacaagga ttacaaagat gacgacgata agcttatgga ctacaaggac gacgatgaca
      181 agcatatggg cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat
      241 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
      301 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
      361 gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt
      421 aatatagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
      481 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
      541 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
      601 gccgcacggc gcgaagcaaa aattacggct cctcgctgcg gacctgcgag cagggaaacg
      661 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
      721 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
      781 acagttctca catcacatcc gaacataaac aaccatgggt aaaaagcctg aactcaccgc
      841 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcggct
      901 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
      961 gcgggtaaat agctgcgccg atggtttcta caaag
//
