LOCUS       sequence-73871-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir50-3
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(304..328)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             681..722
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(762..781)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    806..854
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagttaa gctatcaaca agtttgtaca aaaaagcagg
      121 ctctattttt actaattctg aattccataa ttctatattc tgtattcctc cccgccggcc
      181 gctgatatgt ctggtattct tgggtttgaa cttccagcgt tgaacccgca tattagacgt
      241 atcgacggcc ggcggggcat aatggctgct aagtttggag agaccggggg agtcccagag
      301 atgccccagc tttcttgtac aaagtggttg atctagaggg cccgcagtgg ggtcagcttc
      361 taccggggcg gcgtcccggg gtcttggaac tgcatgcaga ctgcctgctt gggaaacata
      421 cttctttata tgcccatatg gacctgctaa gctatggaat gtaaagaagt atgtatctca
      481 ggccgggacc tctctcgccg cactgagggg cactccacac cacgggggcc gccgcggccg
      541 acagccagcc aggcggctgt ggtgggggtg aggctggggc ggcaggctgc ctgggcgttg
      601 gtggacggtc gtgtccccgc cagcattgtt ctgtcacttt gcctgtgtca gcaggtggaa
      661 gtgctcacca gacattcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
      721 cgcgtaccgg ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca
      781 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt
      841 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
      901 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
      961 aagttcgg
//
