LOCUS       sequence-73885-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir40-wt
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(266..290)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             643..684
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(724..743)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    768..816
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagtt aagctatcaa caagtttgta caaaaaagca ggctggtgta aatcagcttg
      121 gctctggtgt ctccgaacct cctgtccgca cctcagtgga tgtatgccat gatgataaga
      181 tatcagaaat cctatcaccg ggtgtacatc agctaaggtg cgggtacagg tgcatttgat
      241 atcaaggtga gttattttta agtgggccca gctttcttgt acaaagtggt tgatctagag
      301 ggcccgcagt ggggtcagct tctaccgggg cggcgtcccg gggtcttgga actgcatgca
      361 gactgcctgc ttgggaaaca tacttcttta tatgcccata tggacctgct aagctatgga
      421 atgtaaagaa gtatgtatct caggccggga cctctctcgc cgcactgagg ggcactccac
      481 accacggggg ccgccgcggc cgacagccag ccaggcggct gtggtggggg tgaggctggg
      541 gcggcaggct gcctgggcgt tggtggacgg tcgtgtcccc gccagcattg ttctgtcact
      601 ttgcctgtgt cagcaggtgg aagtgctcac cagacattcg aaggtaagcc tatccctaac
      661 cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagtttaaac gggggaggct
      721 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
      781 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
      841 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
      901 ccttttcccc accccacccc ccaagtt
//
