LOCUS       sequence-73894-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pMT mir-1-1 reporter hsa-mir-205
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(445..469)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             483..524
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             883..900
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaaaagcagg ctcatatgag cctccttgga ggatgtgatc ctatgggagg ggtaggagta
       61 ttcaggtcct tgacatctcc caaatgtgtg attccgggat gccaaaggcc tttggccagg
      121 taatgcagtg tctacaggct gaggttgaca tgcatcccca ccctctgaga aaaagatcct
      181 cagacaatcc atgtgcttct cttgtccttc attccaccgg agtctgtctc atacccaacc
      241 agatttcagt ggagtgaagt tcaggaggca tggagctgac aaccatgagg cctcggcagc
      301 caccgccacc accgccgccg ccaccaccgt agcagcagca gcagcagcag cagcagcagc
      361 agcagcagca agagtaactc tgacttagga atagagacag ccagagagaa atgtgatcaa
      421 tgaaagagac atctggagaa gcttacccag ctttcttgta caaagtggtt cgatggttcg
      481 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgttct agagcagtgg
      541 ggtcagcttc taccggggcg gcgtcccggg gtcttggaac tgcatgcaga ctgcctgctt
      601 gggaaacata cttctttata tgcccatatg gacctgctaa gctatggaat gtaaagaagt
      661 atgtatctca ggccgggacc tctctcgccg cactgagggg cactccacac cacgggggcc
      721 gccgcggccg acagccagcc aggcggctgt ggtgggggtg aggctggggc ggcaggctgc
      781 ctgggcgttg gtggacggtc gtgtccccga cagcattgtt ctgtcacttt gcctgtgtca
      841 gcaggtggaa gtgctcacca gacatctaga acgcgtaccg gtcatcatca ccatcaccat
      901 tgagtttaaa cccgctgatc agcctcgact g
//
