LOCUS       sequence-74191-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic G9-5FLAG epitope tagging in S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-G9-5FLAG-kanMX6
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..206
                     /codon_start=1
                     /product="five tandem FLAG(R) epitope tags"
                     /label=5xFLAG
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     terminator      233..420
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(288..312)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        495..838
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(906..925)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aagcttcgta cgctgcaggt cgacggatcc ccgggggagg tggaggtgga ggtggaggtt
       61 taattaatca tatggactac aaggacgacg atgacaagga ttacaaagat gacgacgata
      121 agcttatgga ctacaaggac gacgatgaca aggattacaa agatgacgac gataagctta
      181 tggactacaa ggacgacgat gacaagcata tgggcgcgcc acttctaaat aagcgaattt
      241 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
      301 ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta
      361 ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg
      421 cagatccgct agggataaca gggtaatata gatctgttta gcttgcctcg tccccgccgg
      481 gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca
      541 gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat
      601 catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc
      661 tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg
      721 cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac
      781 ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat
      841 gggtaaggaa aagactcacg tttcgaggcc gcgattaaat tccaacatgg atgctgattt
      901 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt
      961 gtatgggaag cccgatgcgc cagagttgtt tctga
//
