LOCUS       sequence-74624-e        9262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes fabD, accA, accB, accC, accD in pseudo-operon 
            configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 9262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pACM4-ACA
FEATURES             Location/Qualifiers
     source          1..9262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     520..539
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(559..636)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     842..861
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    971..995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1010..1032
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1988..2032
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        2070..2088
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2089..2113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2128..2150
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3130..3174
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        3212..3230
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3231..3255
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3270..3292
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3783..3827
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        3865..3883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3884..3908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3923..3945
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5315..5359
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        5397..5415
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5416..5440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5455..5477
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6412..6456
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(6495..6513)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6509..6556
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6681..7340)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7255..7274
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7341..7443)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(7835..7852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7969..8513)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8049..8068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8738..9262,1..558))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
       61 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
      121 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
      181 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
      241 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
      301 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
      361 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
      421 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
      481 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
      541 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
      601 cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat
      661 gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg
      721 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
      781 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
      841 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
      901 cgatgcgtcc ggcgtagcct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
      961 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
     1021 ttaagaagga gatatacata tgacgcaatt tgcatttgtg ttccctggac agggttctca
     1081 aaccgttgga atgctggctg atatggcggc gagctatcca attgtcgaag aaacgtttgc
     1141 tgaagcttct gcggcgctgg gctacgacct gtgggcgctg acccagcagg ggccagctga
     1201 agaactgaat aaaacctggc aaactcagcc tgcgctgttg actgcatctg ttgcgctgta
     1261 tcgcgtatgg cagcagcagg gcggtaaagc accggcaatg atggccggtc acagcctggg
     1321 ggaatactcc gcgctggttt gcgctggtgt gattgatttc gctgatgcgg tgcgtctggt
     1381 tgagatgcgc ggcaagttca tgcaagaagc cgtaccggaa ggcacgggcg ctatggcggc
     1441 aatcatcggt ctggatgatg cgtctattgc gaaagcgtgt gaagaagctg cagaaggtca
     1501 ggtcgtttct ccggtaaact ttaactctcc gggacaggtg gttattgccg gtcataaaga
     1561 agcggttgag cgtgctggcg ctgcctgtaa agcggcgggc gcaaaacgcg cgctgccgtt
     1621 accagtgagc gtaccgtctc actgtgcgct gatgaaacca gcagccgaca aactggcagt
     1681 agaattagcg aaaatcacct ttaacgcacc aacagttcct gttgtgaata acgttgatgt
     1741 gaaatgcgaa accaatggtg atgccatccg tgacgcactg gtacgtcagt tgtataaccc
     1801 ggttcagtgg acgaagtctg ttgagtacat ggcagcgcaa ggcgtagaac atctctatga
     1861 agtcggcccg ggcaaagtgc ttactggcct gacgaaacgc attgtggaca ccctgaccgc
     1921 ctcggcgctg aacgaacctt cagcgatggc agcggcgctc gagctttaag gtaccctcga
     1981 gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag cacatggact cgtctactag
     2041 gatcgagatc gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc
     2101 ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg
     2161 agtctgaatt tccttgattt tgaacagccg attgcagagc tggaagcgaa aatcgattct
     2221 ctgactgcgg ttagccgtca ggatgagaaa ctggatatta acatcgatga agaagtgcat
     2281 cgtctgcgtg aaaaaagcgt agaactgaca cgtaaaatct tcgccgatct cggtgcatgg
     2341 cagattgcgc aactggcacg ccatccacag cgtccttata ccctggatta cgttcgcctg
     2401 gcatttgatg aatttgacga actggctggc gaccgcgcgt atgcagacga taaagctatc
     2461 gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga tcattggtca tcaaaaaggt
     2521 cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga aggttaccgc
     2581 aaagcactgc gtctgatgca aatggctgaa cgctttaaga tgcctatcat cacctttatc
     2641 gacaccccgg gggcttatcc tggcgtgggc gcagaagagc gtggtcagtc tgaagccatt
     2701 gcacgcaacc tgcgtgaaat gtctcgcctc ggcgtaccgg tagtttgtac ggttatcggt
     2761 gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg ataaagtgaa tatgctgcaa
     2821 tacagcacct attccgttat ctcgccggaa ggttgtgcgt ccattctgtg gaagagcgcc
     2881 gacaaagcgc cgctggcggc tgaagcgatg ggtatcattg ctccgcgtct gaaagaactg
     2941 aaactgatcg actccatcat cccggaacca ctgggtggtg ctcaccgtaa cccggaagcg
     3001 atggcggcat cgttgaaagc gcaactgctg gcggatctgg ccgatctcga cgtgttaagc
     3061 actgaagatt taaaaaatcg tcgttatcag cgcctgatga gctacggtta cgcgtaactc
     3121 gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc agcacatgga ctcgtctact
     3181 aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga
     3241 gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata
     3301 tggatattcg taagattaaa aaactgatcg agctggttga agaatcaggc atctccgaac
     3361 tggaaatttc tgaaggcgaa gagtcagtac gcattagccg tgcagctcct gccgcaagtt
     3421 tccctgtgat gcaacaagct tacgctgcac caatgatgca gcagccagct caatctaacg
     3481 cagccgctcc ggcgaccgtt ccttccatgg aagcgccagc agcagcggaa atcagtggtc
     3541 acatcgtacg ttccccgatg gttggtactt tctaccgcac cccaagcccg gacgcaaaag
     3601 cgttcatcga agtgggtcag aaagtcaacg tgggcgatac cctgtgcatc gttgaagcca
     3661 tgaaaatgat gaaccagatc gaagcggaca aatccggtac cgtgaaagca attctggtcg
     3721 aaagtggaca accggtagaa tttgacgagc cgctggtcgt catcgagtaa ctcgagtctg
     3781 gtaaagaaac cgctgctgcg aaatttgaac gccagcacat ggactcgtct actaggatcg
     3841 agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata
     3901 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgctgga
     3961 taaaattgtt attgccaacc gcggcgagat tgcattgcgt attcttcgtg cctgtaaaga
     4021 actgggcatc aagactgtcg ctgtgcactc cagcgcggat cgcgatctaa aacacgtatt
     4081 actggcagat gaaacggtct gtattggccc tgctccgtca gtaaaaagtt atctgaacat
     4141 cccggcaatc atcagcgccg ctgaaatcac cggcgcagta gcaatccatc cgggttacgg
     4201 cttcctctcc gagaacgcca actttgccga gcaggttgaa cgctccggct ttatcttcat
     4261 tggcccgaaa gcagaaacca ttcgcctgat gggcgacaaa gtatccgcaa tcgcggcgat
     4321 gaaaaaagcg ggcgtccctt gcgtaccggg ttctgacggc ccgctgggcg acgatatgga
     4381 taaaaaccgt gccattgcta aacgcattgg ttatccggtg attatcaaag cctccggcgg
     4441 cggcggcggt cgcggtatgc gcgtagtgcg cggcgacgct gaactggcac aatccatctc
     4501 catgacccgt gcggaagcga aagctgcttt cagcaacgat atggtttaca tggagaaata
     4561 cctggaaaat cctcgccacg tcgagattca ggtactggct gacggtcagg gcaacgctat
     4621 ctatctggcg gaacgtgact gctccatgca acgccgccac cagaaagtgg tcgaagaagc
     4681 gccagcaccg ggcattaccc cggaactgcg tcgctacatc ggcgaacgtt gcgctaaagc
     4741 gtgtgttgat atcggctatc gcggtgcagg tactttcgag ttcctgttcg aaaacggcga
     4801 gttctatttc atcgaaatga acacccgtat tcaggtagaa cacccggtta cagaaatgat
     4861 caccggcgtt gacctgatca aagaacagct gcgtatcgct gccggtcaac cgctgtcgat
     4921 caagcaagaa gaagttcacg ttcgcggcca tgcggtggaa tgtcgtatca acgccgaaga
     4981 tccgaacacc ttcctgccaa gtccgggcaa aatcacccgt ttccacgcac ctggcggttt
     5041 tggcgtacgt tgggagtctc atatctacgc gggctacacc gtaccgccgt actatgactc
     5101 aatgatcggt aagctgattt gctacggtga aaaccgtgac gtggcgattg cccgcatgaa
     5161 gaatgcgctg caggagctga tcatcgacgg tatcaaaacc aacgttgatc tgcagatccg
     5221 catcatgaat gacgagaact tccagcatgg tggcactaac atccactatc tggagaaaaa
     5281 actcggtctt caggaaaaat aactcgagtc tggtaaagaa accgctgctg cgaaatttga
     5341 acgccagcac atggactcgt ctactaggat cgagatcgat ctcgatcccg cgaaattaat
     5401 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
     5461 taactttaag aaggagatat acatatgagc tggattgaac gaattaaaag caacattact
     5521 cccacccgca aggcgagcat tcctgaaggg gtgtggacta agtgtgatag ctgcggtcag
     5581 gttttatacc gcgctgagct ggaacgtaat cttgaggtct gtccgaagtg tgaccatcac
     5641 atgcgtatga cagcgcgtaa tcgcctgcat agcctgttag atgaaggaag ccttgtggag
     5701 ctgggtagcg agcttgagcc gaaagatgtg ctgaagtttc gtgactccaa gaagtataaa
     5761 gaccgtctgg catctgcgca gaaagaaacc ggcgaaaaag atgcgctggt ggtgatgaaa
     5821 ggcactctgt atggaatgcc ggttgtcgct gcggcattcg agttcgcctt tatgggcggt
     5881 tcaatggggt ctgttgtggg tgcacgtttc gtgcgtgccg ttgagcaggc gctggaagat
     5941 aactgcccgc tgatctgctt ctccgcctct ggtggcgcac gtatgcagga agcactgatg
     6001 tcgctgatgc agatggcgaa aacctctgcg gcactggcaa aaatgcagga gcgcggcttg
     6061 ccgtacatct ccgtgctgac cgacccgacg atgggcggtg tttctgcaag tttcgccatg
     6121 ctgggcgatc tcaacatcgc tgaaccgaaa gcgttaatcg gctttgccgg tccgcgtgtt
     6181 atcgaacaga ccgttcgcga aaaactgccg cctggattcc agcgcagtga attcctgatc
     6241 gagaaaggcg cgatcgacat gatcgtccgt cgtccggaaa tgcgcctgaa actggcgagc
     6301 attctggcga agttgatgaa tctgccagcg ccgaatcctg aagcgccgcg tgaaggcgta
     6361 gtggtacccc cggtaccgga tcaggaacct gaggcctgac tcgagtctgg taaagaaacc
     6421 gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagtcgcag cttaattaac
     6481 ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     6541 cttgaggggt tttttgctag cgaaaggagg agtcgaccgg gtcgaatttg ctttcgaatt
     6601 tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac
     6661 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     6721 tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc
     6781 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatagtgaa aacgggggcg
     6841 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     6901 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     6961 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     7021 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     7081 ctatcccata tcaccagctc accgtctttc attgccatac ggaactccgg atgagcattc
     7141 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     7201 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     7261 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     7321 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     7381 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     7441 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca
     7501 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg
     7561 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
     7621 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
     7681 caaaagcacc gccggacatc agcgcaagcg gagtgtatac tggcttacta tgttggcact
     7741 gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc
     7801 agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg
     7861 tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg
     7921 ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct
     7981 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
     8041 aggactataa agataccagg cgtttcccct ggcggctccc tcgtgcgctc tcctgttcct
     8101 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
     8161 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
     8221 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
     8281 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt
     8341 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc
     8401 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg
     8461 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc
     8521 atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag
     8581 cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca tgccccgcgc
     8641 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
     8701 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8761 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8821 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
     8881 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     8941 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
     9001 cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     9061 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
     9121 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
     9181 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
     9241 ccgagacaga acttaatggg cc
//
