LOCUS       sequence-74652-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning insect cell expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Weinert C, Morger D, Djekic A, Grutter MG, Mittl PR
  TITLE     Crystal structure of TRIM20 C-terminal coiled-coil/B30.2 fragment: 
            implications for the recognition of higher order oligomers.
  JOURNAL   Sci Rep. 2015 Jun 4;5:10819. doi: 10.1038/srep10819.
  PUBMED    26043233
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-4"; volume: "5:10819. doi"; pages: " 
            10.1038/srep10819"
COMMENT     Sequence Label: pFBXC3GH
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(85..91,92..109,110..115))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(140..264)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(283..303)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(307..327)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             354..377
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(411..439)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
      121 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
      181 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      241 ttcagctttt ttgtacaaac ttgtgatatc gaattcgcta gctacctttc tcttcttttt
      301 tggatctacc tttctcttct tttttgggga tccactagtt cagctcttct gcattagaag
      361 ttttgtttca aggtccacaa ttcagcaaag gagaagaact tttcactgga gttgtcccaa
      421 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      481 aaggtgatgc tacatacgga aaactcaccc ttaaatttat ttgcactact ggaaaactac
      541 ctgttccttg gccaacactt gtcactactc tgacctatgg tgttcaatgc ttttcccgtt
      601 atccggatca catgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac
      661 aggaacgcac tatatctttc aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt
      721 ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg tattgatttt aaagaagatg
      781 gaaacattct cggacacaaa ctagagtaca acta
//
