LOCUS       sequence-74681-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pNAND-PhlF
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             37..48
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(207..234)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(250..321)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtgaacagc tcctcgccct tgctcaccat ctagtatttc tcctctttct ctagtaaaat
       61 atagtgactg gtctattatc ttgattcgac caccagttgt caattggtaa cgaatcagac
      121 ctagtgaatg agcgacctta acgatacggt acgtttcgta tcatgtcaat tggtaacgaa
      181 tcagattcca ccgtacgtcg tataaacgca gaaaggccca cccgaaggtg agccagtgtg
      241 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      301 ctgagccttt cgttttattt gatgcctggg ggtatgggcc ctagttaacg ctgtgtaccc
      361 ggacaaacac cattaatcag caggaaggta aattcttcaa tatcctgttc aacggtcagc
      421 tgttcggtca gcaggcgata ccaacaaaaa ccaaaaatca tatccagcag cagttcacga
      481 ttggtatctt tcggcagttc accattgcta atggcatttt caaccagttt tttcggcatc
      541 tcacgacgac gttccataaa ctgatctttc agctgggtca gggttgcagg gtccagctgt
      601 gcttctgcaa taacacaacg aaatgcttca ccacaaatgg tttcacgcca aactttccac
      661 agattacgca gcagaaaatc cagatcggct ttaaagctac ccagatccgg aaatttacgc
      721 acctgttcgc tttcattttc atacacttcg gcaatcagtg ctgctttatt ggtccaccaa
      781 cgataaatgg tcggtttgct tgcaccggca cgacgtgcaa cgctttcaat gctcagaccg
      841 ctataaccac attctttcag gatttcaatg gtgctggtca gaattgcttt atgggtatgc
      901 ggactacgca ggctaccaat gctgctacgg ctcggggtac gtgccatcta gtatttctcc
      961 ctttcaaaca tagtccatag aattgt
//
