LOCUS       sequence-74707-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDIRE
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..28
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catggtgccc aagaagaaga ggaaagtctc caacctgctg actgtgcacc aaaacctgcc
       61 tgccctccct gtggatgcca cctctgatga agtcaggaag aacctgatgg acatgttcag
      121 ggacaggcag gccttctctg aacacacctg gaagatgctc ctgtctgtgt gcagatcctg
      181 ggctgcctgg tgcaagctga acaacaggaa atggttccct gctgaacctg aggatgtgag
      241 ggactacctc ctgtacctgc aagccagagg cctggctgtg aagaccatcc aacagcacct
      301 gggccagctc aacatgctgc acaggagatc tggcctgcct cgcccttctg actccaatgc
      361 tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg agagagccaa
      421 gcaggccctg gcctttgaac gcactgactt tgaccaagtc agatccctga tggagaactc
      481 tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca acaccctgct
      541 gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg atggtgggag
      601 aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg tggagaaggc
      661 cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg gtgtggctga
      721 tgaccccaac aactacctgt tctgccgggt cagaaagaat ggtgtggctg ccccttctgc
      781 cacctcccaa ctgtccaccc gggccctgga agggatctt
//
