LOCUS       sequence-75105-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Sox2 fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLPS-hSox2-gfp
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    232..265
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(360..379)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gctcctcgcc cttgctcgcg accggtagct gtggaaaaaa aagggacagg ataagtatga
       61 tattgtcaag gaaaccctga attcgaagct tgagctcgag atccacgtca ggtggcactt
      121 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      181 atccgctcat gagacaataa ccctgataaa tgcttcaata atattggatc tataacttcg
      241 tataatgtat gctatacgaa gttatggatc tttcaggagc taaggaagct aaaatggaga
      301 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      361 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      421 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      481 ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg
      541 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      601 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      661 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      721 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      781 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      841 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      901 gaatgcttaa tgaattacaa cagtactgcg atga
//
