LOCUS       sequence-75110-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Bassett AR, Tibbit C, Ponting CP, Liu JL
  TITLE     Mutagenesis and homologous recombination in Drosophila cell lines 
            using CRISPR/Cas9.
  JOURNAL   Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi: 
            10.1242/bio.20137120.
  PUBMED    24326186
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2013 Dec 10. pii: bio.20137120v1. doi: 10.1242/bio.20137120."
COMMENT     Sequence Label: pAc-y1sgRNA-Cas9
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..103
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             110..130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcggggtacc tactagtcca gtgtggtgga attcatggac tataaggacc acgacggaga
       61 ctacaaggat catgatattg attacaaaga cgatgacgat aagatggccc caaagaagaa
      121 gcggaaggtc ggtatccacg gagtcccagc agccgacaag aagtacagca tcggcctgga
      181 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
      241 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
      301 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
      361 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
      421 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
      481 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
      541 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
      601 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
      661 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
      721 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
      781 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
      841 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
      901 caacttcaag agcaacttcg acctgg
//
