LOCUS       sequence-75113-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Bassett AR, Tibbit C, Ponting CP, Liu JL
  TITLE     Mutagenesis and homologous recombination in Drosophila cell lines 
            using CRISPR/Cas9.
  JOURNAL   Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi: 
            10.1242/bio.20137120.
  PUBMED    24326186
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2013 Dec 10. pii: bio.20137120v1. doi: 10.1242/bio.20137120."
COMMENT     Sequence Label: pAc-y1sgRNA-Cas9
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(130..205)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(226..625)
                     /label=dU6-2 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-2 
                     snRNA (Das et al., 1987)"
ORIGIN
        1 tctatattct aaaaacacaa atgatacttc taaaaaaaaa tcatgaatgg catcaactct
       61 gaatcaaatc tttgcagatg cacctacttc tcatttccac tgtcacatca tttttccaga
      121 tctaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt
      181 aacttgctat ttctagctct aaaaccggtt ccagtgtcca aaaccgaagt attgaggaaa
      241 acatacctat ataaatgatc aacatcagga aagagcagtt gagaattata agaattggca
      301 aatggtctta agaaccctct gcttaagatt ttcaaaattt cctttagaat caaagtgtcc
      361 tattgttcgt ttgttgaaaa cacagttcga tttattgact ataataaatt gatagtttta
      421 aatatagagg cacgactaag agagtttggt tttgttttgg ttagggacca aaaaaagtat
      481 acataacgaa taaaaaagga tttaagtgca aatggtaaaa aagtgtcgag tttttcttga
      541 gttgattgtg ctgtaatgag actctgcatt cggcatttga ctcggctttt cctactcgtg
      601 ccgtatttca ggctgcaagt cgaacagatc tcgctgcctg ttatgtggcc cacaaaccaa
      661 gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat acataatgaa
      721 tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag taggagaatg
      781 cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc caactgacca
      841 actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc gctccattct
      901 tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg gcaagataag
      961 attgtgtc
//
