LOCUS       sequence-75162-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Blumenthal A, Trujillo C, Ehrt S, Schnappinger D
  TITLE     Simultaneous analysis of multiple Mycobacterium tuberculosis 
            knockdown mutants in vitro and in vivo.
  JOURNAL   PLoS One. 2010 Dec 22;5(12):e15667. doi: 
            10.1371/journal.pone.0015667.
  PUBMED    21203517
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-22"; volume: "5(12):e15667. doi"; pages: " 
            10.1371/journal.pone.0015667"
COMMENT     Sequence Label: pDO23A
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(36..138)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    296..527
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(565..582)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(565..581)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(574..596)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      645..731
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
       61 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
      121 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
      181 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
      241 tcggcgcaaa gtgcgtcggg tgatgctgcc aacttagtcg acgctctaga gctccaaata
      301 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
      361 tttataatgc caactttgta caagaaagtt gaacgagaaa cgtaaaatga tataaatatc
      421 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
      481 atgcagtcac tatgaaccaa ctacttagat ggtattagtg acctgtagaa ttcgagctct
      541 agagctgcag ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa
      601 catccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
      661 ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
      721 gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc ggaagggtgg
      781 cgggcagga
//
