LOCUS       sequence-75204-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB66
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cttgtagagc tcgtccattc cgtgggtgat tccggcagcg gtgacgaact ccttgaggac
       61 catgtggtca cgcttctcgt ttgggtcctt ggagagggcg gattgggtgg agaggtagtg
      121 gttgtctggg aggaggactg gtccgtctcc gattggggtg ttttgttggt agtggtcggc
      181 gagttggaca gatccgtcct cgatgttgtg acggatctga aaatttaaat cagattagtt
      241 agttagtaaa atcatgttta aacttacctt gaagttgacc ttgattccgt tcttttgctt
      301 gtcggccatg atgtagacgt tgtgggagtt gtagttgtat tcgagcttgt gtccgaggat
      361 gtttccgtcc tccttgaagt cgattccctg aaaatttaaa tatgtatggt tagttagtac
      421 cgaactgttt aaacttacct tgagctcgat acggttgacg agggtgtctc cctcgaactt
      481 gacctcggca cgggtcttgt agtttccgtc gtcctgaaaa tttaaataat cagggttagt
      541 tagtattaat taatgtttaa acttaccttg aagaagatgg tacgctcttg gacgtatccc
      601 tctggcatgg cggacttgaa gaagtcgtga cgcttcatgt ggtctgggta acggg
//
