LOCUS       sequence-75238-e         455 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for cloning spacer into sgRNA for NmCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 455)
  TITLE     Thomson/Sontheimer Lab NmCas9 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSmart-Nm-sgRNA-BbsI
FEATURES             Location/Qualifiers
     source          1..455
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(9..40)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     misc_RNA        complement(120..212)
                     /label=Nm tracrRNA
                     /note="trans-activating CRISPR RNA for the Neisseria 
                     meningitidis CRISPR/Cas9 system (Hou et al., 2013)"
     primer_bind     complement(321..340)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gccgatcaag tcaaaagcct ccggtcggag gcttttgact ttctgctatg gaggtcaggt
       61 atgatttaaa tggtcagtat tgagcgatat ctagagaatt cgtcgcaaaa aaaccgaaat
      121 aaacgatgcc ccttaaagca gaagctttaa ggggcagagc gttgcggcac atcttttcag
      181 acggccttat tgtagcaacg gttctcattt cgtttccgaa atgagaaagg gagctacaac
      241 ttgtcttctg gaatgaagac ccggtgtttc gtcctttcca caagatatat aaagccaaga
      301 aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta
      361 aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct
      421 ttgtgtttac agtcaaatta attctaatta tctct
//
