LOCUS       sequence-75246-e         554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Gurtan AM, Ravi A, Rahl PB, Bosson AD, JnBaptiste CK, Bhutkar A, 
            Whittaker CA, Young RA, Sharp PA
  TITLE     Let-7 represses Nr6a1 and a mid-gestation developmental program in 
            adult fibroblasts.
  JOURNAL   Genes Dev. 2013 Apr 15;27(8):941-54. doi: 10.1101/gad.215376.113.
  PUBMED    23630078
REFERENCE   2  (bases 1 to 554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.215376"; journalName: "Genes Dev"; date: "2013-04-15- 
            15"; volume: "27"; issue: "8"; pages: "941-54"
COMMENT     Sequence Label: pMMP-puro-Flag-HA-NR6A1
FEATURES             Location/Qualifiers
     source          1..554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(304..330)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(331..354)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(409..425)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 gccctcgcag gagatgatcc catagtgcaa gcccgtagcg cggtccccac agatgagaca
       61 ggttcgttgt tcagctcgat catctgggac ggaaacaggt atatggcctt ggccaagtgt
      121 taaactgtca gtctctccat tagtgcctgg atcaagctct gccaattcat cctgacagaa
      181 accgttgcgc ggcggcggag ggagcgcggc gggcggctcc aggaaccccg ccgagccccc
      241 gccgcctccc cctccgctag gtggccgttc gtcccgctct ccggcgccct caccgcgtgc
      301 gctagcgtaa tctggtacgt cgtatgggta cttatcgtcg tcatccttgt aatcactcat
      361 ggcagtctag aggatggtcc acccccgggg tcggcagcct tcacgtgggc ggcgtgtatc
      421 caagctgcga tgccgtctac tttgagggcg gtgggggtgg tcagcaggac tgtgtaaggt
      481 cctttccagc gaggttctag gttcttagtc tggtgtcngg cggacccaca ctgtgtcgcc
      541 gactcggtaa gggt
//
