LOCUS       sequence-75273-e         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid (Gateway cloning) for cumate-inducible 
            expression of myc-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQMD
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(101..107,108..125,126..131))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(156..280)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(305..334)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    359..386
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    429..456
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
ORIGIN
        1 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
       61 gctcctgaaa atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca
      121 taaagtgtaa agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt
      181 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      241 cattttacgt ttctcgttca gcttttttgt acaaacttgt ctctagagtc atattaatcg
      301 agcccaggtc ctcttccgag atcagcttct gctccatgcg gaagcttcct ctactagtta
      361 caaacagacc agattgtctg tttgttaacc ggcgcagtta gcggcttcgc ggcgatccgt
      421 caaccatgta caaacagacc agattgtctg tttgttccgg tacattgaca tatgtatccg
      481 ctcatgatac aataaccctg atgctagcag ggagagaccc cgaatggtca tcatgtcgcc
      541 gaagcgccgc acccaggccg agcgcgcgat ggaaacccag ggcaagctga tcgccgccgc
      601 cctgggcgtc ctgcgcgaga agggctatgc cggcttccgc atcgccgacg tcccgggc
//
