LOCUS       sequence-75352-e        8567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system, 
            for cloning of TALENs and expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8567)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALEN(Nd154C63) SFFV
FEATURES             Location/Qualifiers
     source          1..8567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..235
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    463..588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2621..3022
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3027..3728
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MASELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     CDS             3732..3785
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3792..3818
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3822..3842
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4143..4550)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     4400..4422
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4414..4431
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4415..4431
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4441..4460
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4441..4459
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4507..4527)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4507..4525)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4546..4562)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4546..4562)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4559..4581)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4570..4586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4594..4600,4601..4618,4619..4624))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4639..4660
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             4852..5439
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    complement(6106..6139)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6344..6361)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6350..6574
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6647..6664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6818..7406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6898..6917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7577..8368,8369..8437))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8200..8219
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8438..8542)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aatgaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg attacgcgtg tggcatctga agcaccacca gcgagcgaga gctagagaga
     1981 aggaaagcca ccgacttcac cgcctccgag ctgctccggg tcgcgggtct gcagcgtcta
     2041 cggccctccg cgcctacagc tcaagccaca tccgaagggg gagggagccg ggagctgcgc
     2101 gcggggccgc cggggggagg ggtggcaccg cccacgccgg gcggccacga agggcggggc
     2161 agcgggcgcg cgcccggcgg ggggaggggc cgcgcgccgc gcccgctggg aattggggcc
     2221 ctagggggag ggcggaggcg ccgacgaccg cggcacttac cgttcgcggc gtggcgcccg
     2281 gtggtcccca aggggaggga agggggaggc ggggcgagga cagtgaccgg agtctcctca
     2341 gcggtggctt ttctgcttgg cagcctcagc ggctggcgcc aaaaccggac tccgcccact
     2401 tcctcgcccc tgcggtgcga gggtgtggaa tcctccagac gctgggggag ggggagttgg
     2461 gagcttaaaa actagtaccc ctttgggacc actttcagca gcgaactctc ctgtacacca
     2521 ggggtcagtt ccacagacgc gggccagggg tgggtcattg cggcgtgaac aataatttga
     2581 ctagaagttg attcgggtgt ttccggaatt cctagctgca gtaacgccat tttgcaaggc
     2641 atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta catgaaaata
     2701 gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg cccggggcca
     2761 agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga tggtccccag
     2821 atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg ctcccccaag
     2881 gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt
     2941 tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac tcggcgcgcc
     3001 agtcctccga cagactgagt cgcccgatgg ctagcgagct gattaaggag aacatgcaca
     3061 tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg
     3121 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc
     3181 tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca
     3241 accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg
     3301 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     3361 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc
     3421 ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacactg taccccgctg
     3481 acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga
     3541 tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg
     3601 gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg
     3661 tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca
     3721 agcttaattc agagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatccgg
     3781 gcccctgcag gtatccatat gatgtcccag attatgcgcc acctaagaag aaacgcaaag
     3841 tcgtggatct acgcacgctc ggctacagtc agcagcagca agagaagatc aaaccgaagg
     3901 tgcgttcgac agtggcgcag caccacgagg cactggtggg ccatgggttt acacacgcgc
     3961 acatcgttgc gctcagccaa cacccggcag cgttagggac cgtcgctgtc acgtatcagc
     4021 acataatcac ggcgttgcca gaggcgacac acgaagacat cgttggcgtc ggcaaacagt
     4081 ggtccggcgc acgcgccctg gaggccttgc tcacggatgc gggggagttg agaggtccgc
     4141 cgttacagtt ggacacaggc caacttgtga agattgcaaa acgtggcggc gtgaccgcaa
     4201 tggaggcagt gcatgcatcg cgcaatgcac tgacgggtgc ccccctggag acgggcgccg
     4261 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     4321 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     4381 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
     4441 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cctccagctt
     4501 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     4561 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4621 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4681 cgctttccac cggtcgtctc caacgaccac ctcgtcgcct tggcctgcct cggcggacgt
     4741 cctgccatgg atgcagtgaa aaagggattg ccgcacgcgc cggaattgat cagaagagtc
     4801 aatcgccgta ttggcgaacg cacgtcccat cgcgttgcga tatctagatc ccagctagtg
     4861 aaatctgaat tggaagagaa gaaatctgaa cttagacata aattgaaata tgtgccacat
     4921 gaatatattg aattgattga aatcgcaaga aattcaactc aggatagaat ccttgaaatg
     4981 aaggtgatgg agttctttat gaaggtttat ggttatcgtg gtaaacattt gggtggatca
     5041 aggaaaccag acggagcaat ttatactgtc ggatctccta ttgattacgg tgtgatcgtt
     5101 gatactaagg catattcagg aggttataat cttccaattg gtcaagcaga tgaaatgcaa
     5161 agatatgtcg aagagaatca aacaagaaac aagcatatca accctaatga atggtggaaa
     5221 gtctatccat cttcagtaac agaatttaag ttcttgtttg tgagtggtca tttcaaagga
     5281 aactacaaag ctcagcttac aagattgaat catatcacta attgtaatgg agctgttctt
     5341 agtgtagaag agcttttgat tggtggagaa atgattaaag ctggtacatt gacacttgag
     5401 gaagtgagaa ggaaatttaa taacggtgag ataaactttt aagtcgaccg agcatcttac
     5461 cgccatttat acccatattt gttctgtttt tcttgatttg ggtatacatt taaatgttaa
     5521 tagaacaaaa tggtggggca atcatttaca tttttaggga tatgtaatta ctagttcagg
     5581 tgtattgcca caagacaaac atgttaagaa actttcccgt tatttacgct ctgttcctgt
     5641 taatcaacct ctggattaca aaatttgtga aagattgact gatattctta actatgttgc
     5701 tccttttacg ctgtgtggat atgctgcttt atagcctctg tatctagcta ttgcttcccg
     5761 tacggctttc gttttctcct ccttgtataa atcctggttg ctgtctcttt tagaggagtt
     5821 gtggcccgtt gtccgtcaac gtggcgtggt gtgctctgtg tttgctgacg caacccccac
     5881 tggctggggc attgccacca cctgtcaact cctttctggg actttcgctt tccccctccc
     5941 gatcgccacg gcagaactca tcgccgcctg ccttgcccgc tgctggacag gggctaggtt
     6001 gctgggcact gataattccg tggtgttgtc atcgaattcg gtaccttttt aaaagaaaag
     6061 gggggactgg aagggctaat tcactcccaa cgaagacaag atatcataac ttcgtatagc
     6121 atacattata cgaagttata atttatttgt gaaatttgtg atgctattgc tttatttgta
     6181 accatatgtt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attgcttttt
     6241 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6301 gggaacccac tgcttaagcc tcaataaagc ttgcctcgac cagcctcgac tgtgccttct
     6361 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6421 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6481 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6541 agcaggcatg ctggggatgc ggtgggctct atggcctgca gctgcattaa tgaatcggcc
     6601 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6661 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6721 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6781 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6841 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6901 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6961 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7021 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7081 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7141 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7201 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7261 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7321 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7381 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7441 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7501 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7561 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7621 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7681 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7741 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7801 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7861 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7921 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7981 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8041 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8101 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8161 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8221 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8281 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8341 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8401 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8461 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8521 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacct
//
