LOCUS       sequence-75376-e         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  TITLE     Viral vectors.
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMIG
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(32..40,41..43,44..618)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(205..224)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     434..451
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cggtgaacag ctcctcgccc ttgctcacca tggttgtggc catattatca tcgtgttttt
       61 caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa
      121 acacatgtaa agcatgtgca ccgaggcccc agatcagatc ccatacaatg gggtaccttc
      181 tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca
      241 actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca
      301 cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg
      361 gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca
      421 ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag
      481 aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat
      541 aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg
      601 gggggggagg gagagggggg g
//
