LOCUS       sequence-75377-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pLJM1-EGFP
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    97..214
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        263..773
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     692..710
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(795..814)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
      121 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
      181 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
      241 tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt
      301 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
      361 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
      421 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
      481 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
      541 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
      601 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
      661 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
      721 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
      781 caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
      841 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
      901 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
      961 gcgtcgggct cgacatcg
//
