LOCUS       sequence-75398-e        2334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of constitutively active human IKK-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Mercurio F, Zhu H, Murray BW, Shevchenko A, Bennett BL, Li J, Young 
            DB, Barbosa M, Mann M, Manning A, Rao A
  TITLE     IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for 
            NF-kappaB activation.
  JOURNAL   Science. 1997 Oct 31. 278(5339):860-6.
  PUBMED    9346484
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1997 Oct 31. 278(5339):860-6."
COMMENT     Sequence Label: IKK-2 S177E S181E
FEATURES             Location/Qualifiers
     source          1..2334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..61
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctcgttt agtgaccgtc agaattgatc taccatggac tacaaagacg atgacgacaa
       61 gcttgcggcc gcaatgagct ggtcaccttc cctgacaacg cagacatgtg gggcctggga
      121 aatgaaagag cgccttggga cagggggatt tggaaatgtc atccgatggc acaatcagga
      181 aacaggtgag cagattgcca tcaagcagtg ccggcaggag ctcagccccc ggaaccgaga
      241 gcggtggtgc ctggagatcc agatcatgag aaggctgacc caccccaatg tggtggctgc
      301 ccgagatgtc cctgagggga tgcagaactt ggcgcccaat gacctgcccc tgctggccat
      361 ggagtactgc caaggaggag atctccggaa gtacctgaac cagtttgaga actgctgtgg
      421 tctgcgggaa ggtgccatcc tcaccttgct gagtgacatt gcctctgcgc ttagatacct
      481 tcatgaaaac agaatcatcc atcgggatct aaagccagaa aacatcgtcc tgcagcaagg
      541 agaacagagg ttaatacaca aaattattga cctaggatat gccaaggagc tggatcaggg
      601 cgaactttgc acagaattcg tggggaccct gcagtacctg gccccagagc tactggagca
      661 gcagaagtac acagtgaccg tcgactactg gagcttcggc accctggcct ttgagtgcat
      721 cacgggcttc cggcccttcc tccccaactg gcagcccgtg cagtggcatt caaaagtgcg
      781 gcagaagagt gaggtggaca ttgttgttag cgaagacttg aatggaacgg tgaagttttc
      841 aagctcttta ccctacccca ataatcttaa cagtgtcctg gctgagcgac tggagaagtg
      901 gctgcaactg atgctgatgt ggcacccccg acagaggggc acggatccca cgtatgggcc
      961 caatggctgc ttcaaggccc tggatgacat cttaaactta aagttggttc atatcttgaa
     1021 catggtcacg ggcaccatcc acacctaccc tgtgacagag gatgagagtc tgcagagctt
     1081 gaaggccaga atccaacagg acacgggcat cccagaggag gaccaggagc tgctgcagga
     1141 agcgggcctg gcgttgatcc ccgataagcc tgccactcag tgtatttcag acggcaagtt
     1201 aaatgagggc cacacattgg acatggatct tgtttttctc tttgacaaca gtaaaatcac
     1261 ctatgagact cagatctccc cacggcccca acctgaaagt gtcagctgta tccttcaaga
     1321 gcccaagagg aatctcgcct tcttccagct gaggaaggtg tggggccagg tctggcacag
     1381 catccagacc ctgaaggaag attgcaaccg gctgcagcag ggacagcgag ccgccatgat
     1441 gaatctcctc cgaaacaaca gctgcctctc caaaatgaag aattccatgg cttccatgtc
     1501 tcagcagctc aaggccaagt tggatttctt caaaaccagc atccagattg acctggagaa
     1561 gtacagcgag caaaccgagt ttgggatcac atcagataaa ctgctgctgg cctggaggga
     1621 aatggagcag gctgtggagc tctgtgggcg ggagaacgaa gtgaaactcc tggtagaacg
     1681 gatgatggct ctgcagaccg acattgtgga cttacagagg agccccatgg gccggaagca
     1741 ggggggaacg ctggacgacc tagaggagca agcaagggag ctgtacagga gactaaggga
     1801 aaaacctcga gaccagcgaa ctgagggtga cagtcaggaa atggtacggc tgctgcttca
     1861 ggcaattcag agcttcgaga agaaagtgcg agtgatctat acgcagctca gtaaaactgt
     1921 ggtttgcaag cagaaggcgc tggaactgtt gcccaaggtg gaagaggtgg tgagcttaat
     1981 gaatgaggat gagaagactg ttgtccggct gcaggagaag cggcagaagg agctctggaa
     2041 tctcctgaag attgcttgta gcaaggtccg tggtcctgtc agtggaagcc cggatagcat
     2101 gaatgcctct cgacttagcc agcctgggca gctgatgtct cagccctcca cggcctccaa
     2161 cagcttacct gagccagcca agaagagtga agaactggtg gctgaagcac ataacctctg
     2221 caccctgcta gaaaatgcca tacaggacac tgtgagggaa caagaccaga gtttcacggc
     2281 cctagactgg agctggttac agacggaaga agaagagcac agctgcctgg agca
//
