LOCUS       sequence-75422-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses wt Akt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, 
            Turk BE, Yaffe MB, Sabatini DM
  TITLE     mTORC1 phosphorylation sites encode their sensitivity to starvation 
            and rapamycin.
  JOURNAL   Science. 2013 Jul 26;341(6144):1236566. doi: 
            10.1126/science.1236566.
  PUBMED    23888043
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2013-07-26"; volume: "341(6144):1236566. doi"; pages: " 
            10.1126/science.1236566"
COMMENT     Sequence Label: pRK5 HA-GST-Presc-mAkt1 (wt)
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             115..141
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             172..825
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     783..805
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             847..870
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gcctttctct ccacaggtgt ccactcccag gtccaactgc acctcggttc tatcgattga
       61 attcccgaac cgacagtcgg tctcttcacc aaggccattc gcgccaccat gggctacccc
      121 tatgatgtgc ctgactacgc agatctcaat ggtggtggtg gtgggtcgag aatgtcccct
      181 atactaggtt attggaaaat taagggcctt gtgcaaccca ctcgacttct tttggaatat
      241 cttgaagaaa aatatgaaga gcatttgtat gagcgcgatg aaggtgataa atggcgaaac
      301 aaaaagtttg aattgggttt ggagtttccc aatcttcctt attatattga tggtgatgtt
      361 aaattaacac agtctatggc catcatacgt tatatagctg acaagcacaa catgttgggt
      421 ggttgtccaa aagagcgtgc agagatttca atgcttgaag gagcggtttt ggatattaga
      481 tacggtgttt cgagaattgc atatagtaaa gactttgaaa ctctcaaagt tgattttctt
      541 agcaagctac ctgaaatgct gaaaatgttc gaagatcgtt tatgtcataa aacatattta
      601 aatggtgatc atgtaaccca tcctgacttc atgttgtatg acgctcttga tgttgtttta
      661 tacatggacc caatgtgcct ggatgcgttc ccaaaattag tttgttttaa aaaacgtatt
      721 gaagctatcc cacaaattga taagtacttg aaatccagca agtatatagc atggcctttg
      781 cagggctggc aagccacgtt tggtggtggc gaccatcctc caaaaggtgg aggtggtggg
      841 tcgagtctgg aagttctgtt ccaggggccc tggtcgacaa tgaacgacgt agccattgtg
      901 aaggagggct ggctgcacaa acgaggggaa tatattaaaa cctggcggcc acgctacttc
      961 ctcctcaaga acgatggcac ctttattggc tacaagga
//
