LOCUS       sequence-75496-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Kapust RB, Tozser J, Fox JD, Anderson DE, Cherry S, Copeland TD, 
            Waugh DS
  TITLE     Tobacco etch virus protease: mechanism of autolysis and rational 
            design of stable mutants with wild-type catalytic proficiency.
  JOURNAL   Protein Eng. 2001 Dec . 14(12):993-1000.
  PUBMED    11809930
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Eng. 2001 Dec . 14(12):993-1000."
COMMENT     Sequence Label: pRK792
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..34
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             38..58
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             59..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 taacaataac aacaacctcg ggatcgaggg aaggggagaa aatctttatt ttcaaggtca
       61 tcatcatcat catcatcatg gagaaagctt gtttaagggg ccgcgtgatt acaacccgat
      121 atcgagcacc atttgtcatt tgacgaatga atctgatggg cacacaacat cgttgtatgg
      181 tattggattt ggtcccttca tcattacaaa caagcacttg tttagaagaa ataatggaac
      241 actgttggtc caatcactac atggtgtatt caaggtcaag aacaccacga ctttgcaaca
      301 acacctcatt gatgggaggg acatgataat tattcgcatg cctaaggatt tcccaccatt
      361 tcctcaaaag ctgaaattta gagagccaca aagggaagag cgcatatgtc ttgtgacaac
      421 caacttccaa actaagagca tgtctagcat ggtgtcagac actagttgca cattcccttc
      481 atctgatggc atattctgga agcattggat tcaaaccaag gatgggcagt gtggcagtcc
      541 attagtatca actagagatg ggttcattgt tggtatacac tcagcatcga atttcaccaa
      601 cacaaacaat tatttcacaa gcgtgccgaa aaacttcatg gaattgttga caaatcagga
      661 ggcgcagcag tgggttagtg gttggcgatt aaatgctgac tcagtattgt gggggggcca
      721 taaagttttc atgccgaaac ctgaagagcc ttttc
//
