LOCUS       sequence-75500-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Destination vector containing AttR1-AttR3 site. For use 
            with three-way LR reaction with cDNA in entry vector and a marker in
            pBEG R2-L3 plasmids (module 2 and module 3 plasmids)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pLEG(R1-R3)   #BGV149
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(135..258)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    274..862
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(327..347)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(745..756)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac gatatcggcg
      121 cgccaacccc ggcccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
      181 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa
      241 ctttattata catagttggc tagtggcgtt aacaatcaac ctctggatta caaaatttgt
      301 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
      361 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
      421 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
      481 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
      541 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
      601 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
      661 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
      721 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
      781 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
      841 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
      901 gagcaatcac aagtagcaat acagcagcta cc
//
