LOCUS       sequence-75523-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-BetI
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(128..155)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(171..242)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    890..909
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(915..921,922..937,938..943))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtagc
       61 tagcattata ttgaacgtcc aatcaattgt caattggtaa cgaatccatg aatgacggta
      121 ttataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc
      181 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
      241 tgatgcctgg gggctcgcct tacgattaat cggtcggcag atgctgggtg ataaaatgac
      301 gggtcaggct atttgcacgg gttttatcca gcggtttacc gctcagtgct gcacgcagcc
      361 acagaccatc aatcagtgct gccagaccat aacctgcctc ttgtgcctgt tcacgaggca
      421 gttcacgacg aaattcgcta accagattgc tcagcagacg acgactgcta acctgctgca
      481 gacgatacag catcggctga tgcatgctgc ttgcccaaaa tgccagccat gctttcattg
      541 ctgcgctgct aacctgggtt tcatcaaaat taccaccaac aattgcctgc agacgctgtt
      601 ctgcgctacc ctgcggcagt gcatgcagac gattcagaac tgcatcacgc agctggctgg
      661 taatatcacg catggttgct tccagcagac cgtttttatc gcggaaataa tggctaataa
      721 taccggtgct aacaccggca cgacgtgcaa tctgtgcaat ggttgcatca tgcataccaa
      781 cttcattaat tgcttccagg gttgcatcaa tcagctgacg acgacgaatg ctctgcatac
      841 ccagtttcgg cactagtagt tatcctctgt caaatgagca agtcgtagca attgtgagcg
      901 ctcacaattc cacacattat acgagccgat gattaattgt caacacagcc aacgatcgtt
      961 ccgatcagaa caagaaagct atgtggcctg acgaagcgg
//
