LOCUS       sequence-75534-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-IcaR
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(171..242)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    857..876
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(882..888,889..904,905..910))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtaac
       61 aacctaacct aacgaaaggt aggtgaattg tcaattggta acgaatcttc caccgtacgt
      121 cgtataaacg cagaaaggcc cacccgaagg tagccagtgt gactctagta gagagcgttc
      181 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
      241 tgatgcctgg gggtatgggc cctagttatt tcttcagaaa gatgttcagc aggctataaa
      301 cctcatcttt aaactggctt ttgctttctt caactgcacc aaatttctgg ctaaagctgg
      361 ctttcagata ccagctttcc agaaacagca gaatcagatt ctggaagtcc tctttggtca
      421 ttttgatttt gctttcgtca tagaatttgg cgatttcttt gctcaggctc tgattcagat
      481 cctggatctg gccataaatg ttacctgaaa attcttccgg tgtgttgctc agctgaacat
      541 acatacgaat atagcgttcc tcgatatcga agatgaactc aaacagaaac tgatacaggg
      601 catcgatgct atagttgctt ttgttctggt tcatcatgat gatgttgttc agataatcga
      661 aacagcattt aacgctctgc tcgtagatgc tttttttgct atcaaagtga taatacaggc
      721 tggctttttt gatgttcacg ctttttgcaa tatcatccag ggtggtgccg tcataacctt
      781 tttcgctaaa cagggtgatg gcgttatcga taattttgtc tttcatccgg cccctgtgtg
      841 aaaacatagt ccatagaatt gtgagcgctc acaattccac acattatacg agccgatgat
      901 taattgtcaa cacagccaac gatcgttccg atcagaacaa gaaagctatg tggcctgacg
      961 aagcggcgcg ccatcgaatg gcgcaaaacc tttcgcggta
//
