LOCUS       sequence-75559-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for cGMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Bhargava Y, Hampden-Smith K, Chachlaki K, Wood KC, Vernon J, 
            Allerston CK, Batchelor AM, Garthwaite J
  TITLE     Improved genetically-encoded, FlincG-type fluorescent biosensors for
            neural cGMP imaging.
  JOURNAL   Front Mol Neurosci. 2013 Sep 24;6:26. doi: 10.3389/fnmol.2013.00026.
  PUBMED    24068983
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2013-09-24"; volume: "6:26. doi"; pages: " 
            10.3389/fnmol.2013.00026"
COMMENT     Sequence Label: pTriEx4-H6-FGB
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..122
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             170..214
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             230..247
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             266..280
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 gagttaatcc gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta
       61 ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa
      121 tcaaaggaga tataccatgg cacaccatca ccaccatcac tcttctggta aagaaaccgc
      181 tgctgcgaaa tttgaacgcc agcacatgga ctcgccaccg ccttctggtc tggtcccccg
      241 gggcagcgca ggttctggta cgattgatga cgacgacaag atggtactcc gacaggcatt
      301 ccggaagttc accaaatccg aaaggtccaa ggatctcata aaggaggcca tccttgacaa
      361 tgactttatg aagaacttgg agctgtcaca gatccaagag attgtggatt gtatgtaccc
      421 agtggagtac ggcaaagaca gctgcatcat caaagaagga gatgtggggt cactggtgta
      481 tgtcatggaa gatggtaagg ttgaagttac aaaagaaggc gtgaagctgt gcacaatggg
      541 tcctggtaaa gtgtttggag agttggctat cctttacaac tgtacccgga cggcgaccgt
      601 caaaactctt gtaaatgtga aactctgggc cattgatcga caatgttttc agacgataat
      661 gatgaggaca ggacttatca agcataccga gtatatggaa tttttaaaaa gcgttccaac
      721 attccagagc cttcctgaag agatcctcag taagcttgct gacgtccttg aagagaccca
      781 ctatgaaaat ggggaatata tcatcaggca aggtgcaaga ggggacacct tctttatcat
      841 cagtaaagga aaggttaatg tcactcgtga agactcgccc aatgaagacc cagtctttct
      901 tagaacctt
//
