LOCUS       sequence-75617-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged human EAF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-Flag-EAF2
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc atgccagact acaaggacga tgatgacaag
      121 ctcgagatga atagcgcagc gggattctca cacctagacc gtcgcgagcg ggttctcaag
      181 ttaggggaga gtttcgagaa gcagccgcgc tgcgccttcc acactgtgcg ctatgacttc
      241 aaacctgctt ctattgacac ttcttctgaa ggataccttg aggttggtga aggtgaacag
      301 gtgaccataa ctctgccaaa tatagaaggt tcaactccac cagtaactgt tttcaaaggt
      361 tcaaaaaaac cttacttaaa agaatgcatt ttgattatta accatgatac tggagaatgt
      421 cggctagaaa aactcagcag caacatcact gtaaaaaaaa caagagttga aggaagcagt
      481 aaaattcagt atcgtaaaga acaacagcaa caacaaatgt ggaattcagc caggactccc
      541 aatcttgtaa aacattctcc atctgaagat aagatgtccc cagcatctcc aatagatgat
      601 atcgaaagag aactgaaggc agaagctagt ctaatggacc agatgagtag ttgtgatagt
      661 tcatcagatt ccaaaagttc atcatcttca agtagtgagg atagttctag tgactcagaa
      721 gatgaagatt gcaaatcctc tacttctgat acagggaatt gtgtctcagg acatcctacc
      781 atgacacagt acaggattcc tgatatagat gccagtcata atagatttcg agacaacagt
      841 ggccttctga tgaatacttt aagaaatgat ttgcagctga gtgaatcagg aagtgacagt
      901 gatgactgag gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc
      961 ggccgctcga tcgagtcta
//
