LOCUS       sequence-75618-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of luciferase driven by mPer2 promoter fragment 
            containing E-box2 (bases -112 to +98 with respect to transcription 
            start site at +1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Yoo SH, Ko CH, Lowrey PL, Buhr ED, Song EJ, Chang S, Yoo OJ, 
            Yamazaki S, Lee C, Takahashi JS
  TITLE     A noncanonical E-box enhancer drives mouse Period2 circadian 
            oscillations in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2608-13. Epub 2005 Feb 
            7.
  PUBMED    15699353
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2005-02-15"; volume: "102(7)"; pages: 
            "2608-13. Epub 2005 Feb 7"
COMMENT     Sequence Label: pGL3 basic E2
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      323..332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(395..413)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tctctatcga taggtaccga gctcttacgc gtgctagccc cgcggatccg agcgtagctc
       61 tcaggttccg ccccgccagt atgcaaatga ggtggcactc cgaccaatgg cgcgcgcagg
      121 ggcgggctca gcgcgcgcgg tcacgttttc cactatgtga cagcggaggg cgacgcggcg
      181 gcagcggcgc tactgggact agcggctccg ggcggctgcg gcgcaggccg agcgcaccaa
      241 gtgacgggcc gagcaaggga tctagagcag ggctcgagat ctgcgatcta agtaagcttg
      301 gcattccggt actgttggta aagccaccat ggaagacgcc aaaaacataa agaaaggccc
      361 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa
      421 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat
      481 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
      541 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
      601 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
      661 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa
      721 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat
      781 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
      841 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac
      901 aattgcactg atcatgaact cctctggatc tactggtctg cctaa
//
