LOCUS       sequence-75620-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged human Med26-K74A K75A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-F-Med26-K74A K75A
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..119
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctctctg gctactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcacca tgtacccata tgatgtccct gattacgcat
      121 atccttacga cgtaccagac tatgctctcg acatgacagc ggctccggcg tctccgcagc
      181 agatcaggga ccggctgctg caggccatcg acccccagag caacatccgg aacatggtgg
      241 cggtgctgga agtcatctcc agcctggaga aataccctat taccaaagag gcacttgagg
      301 aaacacgact tgggaagctc atcaacgacg tccgcaagaa aaccaagaac gaggagctcg
      361 ccaagcgggc cgcggcgctg ctgcggagct ggcagaagct catcgagccg gcacaccagc
      421 atgaggcggc gctgcggggg ctggcggggg ccaccggctc tgccaacggg ggcgcacaca
      481 actgccggcc ggaggtgggg gcggctggcc cacccaggag catccatgac ctgaagagcc
      541 gcaatgacct ccagaggctg cccgggcagc ggctggacag gctgggcagc cgcaagcgcc
      601 ggggtgacca gcgtgacctc ggccacccag ggccgccacc caaggtctcc aaagctagcc
      661 acgaccccct ggtccccaac tcatcccccc tccccaccaa cgggatcagt gggagtccag
      721 agagcttcgc cagctccctg gatggcagtg ggcatgcagg cccagagggc agccgcctgg
      781 agcgtgacga gaatgacaag cacagtggca agatccccgt caacgccgtg cgaccgcaca
      841 ccagctcccc gggcctgggc aagccccctg gaccctgctt gcagccaaag gcttcggtgc
      901 tgcagcagct ggacagggtg g
//
