LOCUS       sequence-75631-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FLAG-tagged Fbxl21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Yoo SH, Mohawk JA, Siepka SM, Shan Y, Huh SK, Hong HK, Kornblum I, 
            Kumar V, Koike N, Xu M, Nussbaum J, Liu X, Chen Z, Chen ZJ, Green 
            CB, Takahashi JS
  TITLE     Competing E3 ubiquitin ligases govern circadian periodicity by 
            degradation of CRY in nucleus and cytoplasm.
  JOURNAL   Cell. 2013 Feb 28;152(5):1091-105. doi: 10.1016/j.cell.2013.01.055.
  PUBMED    23452855
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1091-105"
COMMENT     Sequence Label: pCMV10-Flag-Fbxl21
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..85
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 accgtcagaa ttaaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
       61 cgattacaag gatgacgatg acaagcttgc ggccgcgaat tcatcgatag atctgatatc
      121 ggtaccgatg aagaggaata atttctctgc cgtgaataag gttgtccagt cgtcaccagt
      181 agtgaaacag cccaaacgtg ggctctgctc ttccctccgc cagacccatg cgctctctgt
      241 ccttctggac tgggggactt tacctcacca tgtcatatta cagatctttc agtaccttcc
      301 tttaatagat cgagcccggg cttcgtccgt gtgcaggaga tggaatgaag tattccacat
      361 ccctgatctt tggaggaagt ttgagtttga gctgaaccaa tcagcaactt cttatttcaa
      421 gtccactcat cccgacctca ttcaacagat catcaaaaag cacgctgcac atcttcagta
      481 tgtcagcttt aaggttgata gcagtactga gtcggcagaa gccgcctgtg atatcctttc
      541 tcagctggta aattgttcta tccagacctt gggattgatt tcaacagcca aaccaagttt
      601 catgaatgtg cccaagtctc actttgtgtc ggcacttacg gtcgtgtttg tcaactcaaa
      661 atcgttatca tcgattaaaa ttgaggacac gccagtggat gacccttccc tgaagattct
      721 tgtggctaac aacagtgaca cccttcgact cctcaaaatg agtagctgtc ctcacgtttc
      781 atctgatggg atcctctgtg tggcggacca ctgtcagggc ctccgagagc tggccctgaa
      841 ttactacatt ctgagcgacg aaattctcct tgcgctctcc agcgagactc acgtgaacct
      901 cgaacatctc cggatagacg ttgtgagtga aaacccgggg ca
//
