LOCUS       sequence-75655-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-SrpR
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(147..174)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(190..261)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    961..980
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtagt
       61 ttacaaacaa acaagcatgt atgtatatac ctaggactga gctagctgtc aattggtaac
      121 gaatcttcca ccgtacgtcg tataaacgca gaaaggccca cccgaaggtg agccagtgtg
      181 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      241 ctgagccttt cgttttattt gatgcctggg ggtatgggcc ctagttattc gaaggatttc
      301 acctgtttaa tctgtgcttc ttcacacagc agaaaacgag gcggttcacg cagcagtgcc
      361 caaaagctac ccagtgcaac tttgataatc tgtgcctgct gatctttgct acgcagacct
      421 tcatacagca gaccggtaat cagaccttta aaaacaccaa tgctggtctg cagatccaga
      481 ttaatcggca gttcaccctg ggtaactgca tgacgcagaa cctgatgaat atactgcaga
      541 aaacgatcgc ttgcctgaac catacgatta tgaatcagac cgctttcatc cagaccctga
      601 taaatcagaa tttcgctaaa ctgtttgctc tgcggactat gaactgcatc cagcattgca
      661 acaacaactg cttcccagct acgttcaata cccagatccg gtgtaaaatc cagttccagc
      721 ggatgctgac ggcttgccag aactgcctgc agaacttcca gtttaccatt aaaatgccaa
      781 taaactgcac cacgggtaac accggcttta cgtgcaatct gatccagggt tgcatcacta
      841 acaccctgtg caacaaaaac ttccagtgct gcatcaataa tacgctgacg ggtttcttct
      901 gcttctgctg cggttttacg tgccatcctg gtatttcctg tgtgaaaaca tagtccatag
      961 aattgtgagc gctcacaatt ccacacatta tacgagccga
//
