LOCUS       sequence-75710-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Left TALEN for PHOX2B with NN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga 
            PP, So PL, Conklin BR
  TITLE     Isolation of single-base genome-edited human iPS cells without 
            antibiotic selection.
  JOURNAL   Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 
            2014 Feb 9.
  PUBMED    24509632
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2840"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "291-3"
COMMENT     Sequence Label: MR015-PHOX2BLeftTALEN_NN
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..160
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             167..187
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgccac catggactac aaagaccatg acggtgatta
      121 taaagatcat gacatcgatt acaaggatga cgatgacaag atggccccca agaagaagag
      181 gaaggtgggc attcaccgcg gggtacctat ggtggacttg aggacactcg gttattcgca
      241 acagcaacag gagaaaatca agcctaaggt caggagcacc gtcgcgcaac accacgaggc
      301 gcttgtgggg catggcttca ctcatgcgca tattgtcgcg ctttcacagc accctgcggc
      361 gcttgggacg gtggctgtca aataccaaga tatgattgcg gccctgcccg aagccacgca
      421 cgaggcaatt gtaggggtcg gtaaacagtg gtcgggagcg cgagcacttg aggcgctgct
      481 gactgtggcg ggtgagctta gggggcctcc gctccagctc gacaccgggc agctgctgaa
      541 gatcgcgaag agagggggag taacagcggt agaggcagtg cacgcctggc gcaatgcgct
      601 caccggtgcc cccctgaacc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg
      661 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
      721 cctgactccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc aagcgctcga
      781 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgactc
//
