LOCUS       sequence-75743-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir129-2-2
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    90..114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(297..321)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             674..715
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(755..774)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    799..847
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag ctggctagtt aagctatcaa caagtttgta caaaaaagca ggctcatcct
      121 gggctgaagg cggcggcgaa ccgaagaagc cggcatattc tgcccttcgc gaatcttttt
      181 gcggtctggg cttgctgtac ataactcaat agccggaagc ccttacccca aaaagcattt
      241 gcggagggcg cactagtaga gaagacggca gccatccagc gatcgccgaa gcccgcaccc
      301 agctttcttg tacaaagtgg ttgatctaga gggcccgcag tggggtcagc ttctaccggg
      361 gcggcgtccc ggggtcttgg aactgcatgc agactgcctg cttgggaaac atacttcttt
      421 atatgcccat atggacctgc taagctatgg aatgtaaaga agtatgtatc tcaggccggg
      481 acctctctcg ccgcactgag gggcactcca caccacgggg gccgccgcgg ccgacagcca
      541 gccaggcggc tgtggtgggg gtgaggctgg ggcggcaggc tgcctgggcg ttggtggacg
      601 gtcgtgtccc cgccagcatt gttctgtcac tttgcctgtg tcagcaggtg gaagtgctca
      661 ccagacattc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
      721 cggttagtaa tgagtttaaa cgggggaggc taactgaaac acggaaggag acaataccgg
      781 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
      841 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
      901 ccccattggg gccaatacgc ccgcgtttct tccttttccc
//
