LOCUS       sequence-75794-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in 
            TVA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A
  TITLE     Dissecting local circuits: parvalbumin interneurons underlie broad 
            feedback control of olfactory bulb output.
  JOURNAL   Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027.
            Epub 2013 Nov 14.
  PUBMED    24239125
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.08.027"; journalName: "Neuron"; date: 
            "2013-12-4- 4"; volume: "80"; issue: "5"; pages: "1232-45"
COMMENT     Sequence Label: CAG-Flex-TC66T
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..74
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    145..178
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      185..194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taatccagag gttgattatc gatcggccgc atataagctt ataacttcgt ataatgtatg
       61 ctatacgaag ttatgggtcg atggtgagat ctggactaga gggtcgatgg tgatgcttgg
      121 atcaaaatag gaagaatccc aatgataact tcgtataaag tatcctatac gaagttatag
      181 gcgcgccacc atggcgcggc tgctgcccgc gctgctgctg ctgctgctgc ccggtaacgt
      241 gaccggtaac gggtccggta acggttcttt gtcccgttgc ccccccggtc agttccgctg
      301 ctcggagccg cccggtgccc acggggagtg ttacccgcag gactggctgt gcgacggaca
      361 ccccgactgc gacgacgggc gggacacctg gggctgcggg accagcgcga tccccgcggt
      421 gcccacggac aacggcacag aggctcccac tgcccctgct cctggacgtg ctctgccagc
      481 caggaatcac ggccgcatgt ggatgctgat cactgcagtg ctcctgtgct gcctggtagc
      541 tgtgggtggt atcgctgcat gggggaagtc caaagcaaaa agcaggtctg acatcttcag
      601 tcttgaaagc gcatccaagg ggggtggggt gagcaagggc gaggaggata acatggccat
      661 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
      721 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg cc
//
