LOCUS       sequence-75811-e         558 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cumate-inducible expression of lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQF-lacZ
FEATURES             Location/Qualifiers
     source          1..558
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..219)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     168..190
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     182..199
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     183..199
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(215..231)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(215..231)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    258..285
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    328..355
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
ORIGIN
        1 gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct
       61 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      121 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      181 ttgtaaaacg acggccagtg aatccgtaat catggtcata gctgtttcct gtgggtaccg
      241 ggatccggac ctctagttac aaacagacca gattgtctgt ttgttaaccg gcgcagttag
      301 cggcttcgcg gcgatccgtc aaccatgtac aaacagacca gattgtctgt ttgttccggt
      361 acattgacat atgtatccgc tcatgataca ataaccctga tgctagcagg gagagacccc
      421 gaatggtcat catgtcgccg aagcgccgca cccaggccga gcgcgcgatg gaaacccagg
      481 gcaagctgat cgccgccgcc ctgggcgtcc tgcgcgagaa gggctatgcc ggcttccgca
      541 tcgccgacgt cccgggcg
//
