LOCUS       sequence-75865-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian SP-VP64 nuclease-null Cas9 activator expression, human 
            optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-SPn-VP64
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc ggactctaga ggatcgaacc cttgccacca
      121 tggacaagaa gtactccatt gggctcgcta tcggcacaaa cagcgtcggc tgggccgtca
      181 ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc
      241 acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag
      301 ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct
      361 acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc
      421 tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca
      481 atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga
      541 agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata
      601 tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg
      661 tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga
      721 tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc
      781 ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc
      841 ttatcgccct gtcactcggg ctga
//
