LOCUS       sequence-75866-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 1,small linker insert, plus 5' 
            ATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_+1_Linker
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          152..724
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaggtct
       61 caatgggcgg atccggcggc ggtaccggct tgagaccctc atcctcccgg gtaactaagt
      121 aaggatctcg accgatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt
      181 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt
      241 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca
      301 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
      361 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
      421 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
      481 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
      541 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt
      601 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
      661 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct
      721 acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattaat
      781 tcctcgacgg atcgta
//
