LOCUS       sequence-75924-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with 
            start and stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_4'_+nls-mCherry-Flag-_+1_BK+
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..46
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     28..45
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             78..98
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(244..262)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     512..531
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     706..729
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctgcctgggg acgtcggagc aagcttgatt taggtgacac tatagaggtc tcaactaccg
       61 gatccgccgc catggctcca aagaagaagc gtaaggtaat ggtgagcaag ggcgaggagg
      121 acaacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      181 acggccgcga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      241 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
      301 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      361 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      421 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      481 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      541 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      601 agaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacaagg
      661 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagctg gacatcacct
      721 cccacaacga ggactacacc atcgtggaac agtacga
//
