LOCUS       sequence-75963-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH45234
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     203..220
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
       61 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      241 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      301 cgggcttgat tcgatattaa tatgggatgg tgtacaacgg gtgaaatttt agaattgact
      361 ggtttgagtt gataataatt tgcgatagaa ctaggagtga aatcctagaa tgacaatcaa
      421 gttattatta tacaattaaa tactaccaca ggtgaaatcc tagaattgac gggtttgatt
      481 cgatattaat atgggatggt gtactacggg tgaaatccga gaaacaacaa tcaaaataca
      541 attatacaat attaaacatt taacaaaata aacaaataca acttaaaact ttaaaatttg
      601 agttataaaa ttttgtctcg cggtgaatta tacatttaaa tcaaacaata gcagaaattt
      661 attaagtcat cctaagaaat attcaattat tttttattta atgaaaatat agacccgcgg
      721 tataccgcgg attaatattt agtgctattg ataaatttat ctcaacgttc agctttgaca
      781 ttgtgaaatg cattatgagt catgactctg tttatagata ttcctgttat
//
