LOCUS       sequence-76012-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH49477
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(158..262)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     330..348
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc
       61 ccaaaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
      121 gttcggtcaa ggttctggac cagttgcgtg agcgcatact cttccttttt caatattatt
      181 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
      241 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
      301 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtccac
      361 tgaagagcca cttcgtggtc tcaaagctta aaggaacaga tggtggcgtc cctcggttct
      421 ttcatactgc tcaacgattg tataatcttc gttgtggctt gttatatcca gtttggagtc
      481 aacatagtag taccctggta gctgcacagg cttctttgcc atgtaaatac ttttgaattc
      541 aactaggtaa tgaccaccgt ctttcagctt cagagcctta tgaatctctc ctttcaacac
      601 gccatcacga ggatacaaac gctcagtgct ggcttcccag cccattgttt tcttttgcat
      661 aacaggtcca tcggaaggaa agttcacgcc aatgaacttg accttgtaga tgaaacagcc
      721 atcctgcaaa ctggaatcct gggttacagt aacgacgcca ccatcttcaa agttcatgac
      781 cctttcccat ttaaatcctt caggaaatga cagcttttta tagtctggta
//
