LOCUS       sequence-76042-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH51288
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(157..261)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     329..347
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        complement(478..524)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(513..533)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
       61 caaaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg
      121 ttcggtcaag gttctggacc agttgcgtga gcgcatactc ttcctttttc aatattattg
      181 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
      241 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
      301 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtccact
      361 gaagagccac ttcgtggtct cacattgtat cgataattgt aaatgtaatt gtaatgttgt
      421 ttgttgtttg ttgttgttgg taattgttgt aaaaatgagc tcttatactc gagcgtgtcc
      481 tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt
      541 gtgcgtcatc ccttacgtca gtggagatgt cacatcaatc cacttgcttt gtagacgtgg
      601 ttggaacctc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
      661 tgtcggcaga gagatcttga atgatagcct ttcctttatc gcaatgatgg catttgtagg
      721 agccaccttc cttttctact gtcctttcga tgaagtgaca gatagctggg caatggaatc
      781 cgaggaggtt
//
