LOCUS       sequence-76131-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial T. denticola Cas9 (TD) + tracrRNA expression, 
            cloDF13/spectinomycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-TDcas
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      273..300
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt
       61 tcaagccgag gggccgcaag agccggccac gatgacccgg tcgtcggttc agggcagggt
      121 accaggcacg cctaaccgtc agtgagattg gatgagtgaa cgatattgat cgagaagagc
      181 cctgcgcagc cgctgccgtg cctgcaggaa gcaacggccc ggagggtggc gggcaggacg
      241 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
      301 tgcgtttcta caaactctgc tagcttctag agcacagcta acaccacgtc gtccctatct
      361 gctgccctag gtctatgagt ggttgctgga taactttacg ggcatgcata aggctcgtat
      421 gatatattca gggagaccac aacggtttcc ctctacaaat aattttgttt aacttttact
      481 agaggaggag gcaaaaatga agaaggagat caaggactac ttcctgggcc tggacgtggg
      541 caccggcagc gtgggctggg ccgtgaccga caccgactac aagctgctga aggccaaccg
      601 caaggacctg tggggcatgc gctgcttcga gactgctgag accgccgagg tgcgccggct
      661 gcaccgcggt gctcgccggc gcatcgagcg ccgcaagaag cgcatcaagc tgctgcagga
      721 gctgttcagc caggagatcg ccaagaccga cgagggcttc ttccagcgca tgaaggagag
      781 ccccttctac gccgaggaca aga
//
