LOCUS       sequence-76134-e         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of untagged gene sequences together 
            with 6 copies of the core DSR motif into the S. pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-6XDSR
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(8..41)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(239..256)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(239..255)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(248..270)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ccagcttata acttcgtata gcatacatta tacgaagtta ttgctagccg ttttctgngn
       61 gtnaantgnn annnnnnatt caatcatatg ctggcatgcg gagagctcgg agtcgacgag
      121 actagtttaa actccgtatt aaacccattc ttaaacagaa ctttaaacgg caggttaaac
      181 gtaatgttaa accccgggat aacttcgtat atggtattat atacgaagtt atgaattcac
      241 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      301 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      361 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
      421 cgcatctgtg cggtatttca caccgcatac catcgtccgg atcaatgaag tagatgagtt
      481 tactacctgt atatactttt ttttttgtca aagtcacttt tatggcattt ccatgttgaa
      541 ggattancca cagcactaaa ttttcagcgt atgattgctt ttaaatattt aattttcatc
      601 gttttttaat taatattcaa aacgatttaa tgccttctac atctgaa
//
